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Molecular biology laboratory student manual molecular cloning biology page 2 return to www101sciencecom home page page 1 index news 1 introduction 2 the scientific method 3 water 4 building a life form 5 at the heart of every scientific or technological discovery is a laboratory technician if youre interested in learning more about how the world works and enjoy research and laboratory analysis . Any basic research laboratory using molecular biology techniques will benefit from having a copy on molecular cloning a laboratory manual fills the same. Molecular cloning has served as the foundation of technical expertise in labs worldwide for 30 years no other manual has been so popular or so influential. From the college board ap biology lab manual student edition 2001 pp64 65 the bacterium escherichia coli e coli is an ideal organism for the molecular geneticist to manipulate and has been used extensively in recombinant dna research. This introductory cloning laboratory is for use in courses that cover basic topics in molec ular biology and genetics molecular cloning is a basic technique used in a molecular biology labs in this manual we include a protocol for isolating the luciferase gene from dna using restriction digestion and cloning it into the multiple cloning region of a vector the cloned luciferase gene is then